ARBOR ASSAYS 免疫測(cè)試產(chǎn)品試劑盒-相關(guān)技術(shù)文章

更新時(shí)間：2024-07-16

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ARBOR ASSAYS 免疫測(cè)試產(chǎn)品試劑盒

Arbor Assays公司成立于2007年，位于密歇根州的安娜堡，公司的三位主創(chuàng)始人均為有多年科研經(jīng)驗(yàn)的生命和臨床科學(xué)家。其宗旨是為臨床使用的重要生物分子建立高質(zhì)量的檢測(cè)和免疫測(cè)試產(chǎn)品。在生物領(lǐng)域建立健全的免疫檢測(cè)和酶活性試劑盒以定量生物分子。

主要為臨床重要生物分子提供高質(zhì)量的檢測(cè)和免疫測(cè)試產(chǎn)品，如乙酰膽堿酯酶活性熒光檢測(cè)試劑盒、谷胱甘肽熒光檢測(cè)試劑盒、血紅蛋白比色檢測(cè)試劑盒等。種類多樣，操作簡(jiǎn)單，靈敏度高。

認(rèn)識(shí)到對(duì)靈敏度、速度、便利性、質(zhì)量和服務(wù)的需求，我們研究、開發(fā)和制造用于臨床重要生物分子的最高質(zhì)量的檢測(cè)和免疫測(cè)定試劑盒。

Arbor Assays公司的主要產(chǎn)品包括：

DetectX® Kits

Inhibitors & Activators

AbX® Antibodies & Affinity Resins

Detection Systems

Components & Ancillary Reagents

所需材料:

類固醇標(biāo)準(zhǔn)品，用于檢測(cè)萃取效率

ACS級(jí)乙醇(或乙酸乙酯)

玻璃試管

處理流程：

確保樣品wan全干燥，并對(duì)樣品進(jìn)行粉末化處理，以提高萃取回收率。如果可能的話清除任何大顆粒，如草屑。我們建議通過使用試劑盒的AB分析緩沖液及已知濃度的類固醇溶液檢測(cè)結(jié)果來確定提取效率，詳細(xì)信息請(qǐng)參考下面的萃取效率部分。乙醇或乙酸乙酯萃取樣品及萃取效率控制如下:

1.稱取≥0.2 g干燥的糞便固體放入管中。樣品可以通過被動(dòng)干燥、溫和加熱(≤60°C)或冷凍的方式來進(jìn)行干燥處理。

2.每0.1 g固體(0.1 g糞便固體/mL)加入1 mL乙醇(或乙酸乙酯)并密封。

3.用力搖晃至少30分鐘。

4.在4°C下，將樣品在5,000 rpm下離心15分鐘。取上清放入一個(gè)干凈的管中。如果密封得當(dāng)，該樣本可在≤-20°C下保存至少一個(gè)月。

注：含有低水平分析物的樣品可以通過干燥提取液和在少量分析緩沖液中重懸，來達(dá)到濃縮的效果。用乙酸乙酯提取的樣品在加入緩沖液之前，必須wan全干燥以去除溶劑。從步驟4中轉(zhuǎn)移測(cè)量體積(蒸發(fā)體積)的上清到一個(gè)干凈的管中，在離心蒸發(fā)裝置或氮?dú)庀抡舭l(fā)至干燥。干燥、提取的樣品可在干燥器中冷凍≤-20°C保存?；蛴靡掖?span>(如100μL)溶解干燥提取的樣品，直接進(jìn)行步驟5。

5.以上步驟為通用步驟，可根據(jù)需要量進(jìn)行等比例體積調(diào)整。將步驟4中提取的樣本上清液100μL加入最少400μL的AB緩沖液中（總重構(gòu)體積=100μL+400μL）。室溫下渦旋混勻，靜置5min，重復(fù)至少兩次渦旋和靜置步驟。對(duì)于免疫分析，測(cè)定中乙醇含量通常需要≤5%，需要根據(jù)所選擇的試劑盒中描述的最大乙醇含量耐受情況，用檢測(cè)緩沖液進(jìn)行稀釋。

6.根據(jù)試劑盒說明書對(duì)稀釋后的樣本進(jìn)行ELISA檢測(cè)。

下面是ARBOR ASSAYS公司的部分熱銷產(chǎn)品

產(chǎn)品名稱	貨號(hào)	規(guī)格
Goat Anti-Mouse IgG Fc, 25MG	A008-25MG	25MG
Cyclic AMP Direct ELISA Kit (1 Plate)	K019-H1	1x96 well plate
Corticosterone ELISA Kit (1 Plate)	K014-H1	1x96 well plate
Protein Kinase A (PKA) Activity Assay Kit (1 Plate)	K027-H1	1x96 well plate
Aldosterone ELISA Kit (1 Plate)	K052-H1	1x96 well plate
Urea Nitrogen (BUN) Detection Kit (2 Plate)	K024-H1	2x96 well plate
Cortisol ELISA Kit (1 Strip plate)	K003-H1	1x96 well plate
PGFM ELISA Kit (1 Plate)	K022-H1	1x96 well plate
Triiodothyronine (T3) ELISA Kit (1 Plate)	K056-H1	1 Kit
FRAP™ (Ferric Reducing Antioxidant Power) Detection Kit (2 Plate)	K043-H1	2x96 well plate
Hemoglobin Detection Reagent, 20ML	C038-20ML	20ML
Ceruloplasmin Colorimetric Activity Kit (2 Plate)	K035-H1	2x96 well plate
ThioStar™ Fluorescent Thiol Substrate, 100UG	L002-100UG	100UG
Cyclic GMP Direct Chemiluminescent ELISA Kit (1 Plate)	K020-C1	1x96 well plate
Progesterone ELISA Kit (1 Plate)	K025-H1	1x96 well plate
Testosterone ELISA Kit (1 Plate)	K032-H1	1x96 well plate
Estrone-3-Glucuronide (E1G) ELISA Kit (1 Plate)	K036-H1	1x96 well plate
Thyroxine (T4) ELISA Kit (1 Plate)	K050-H1	1x96 well plate
Prostaglandin E2 (PGE2) Multi-Format ELISA Kit (1 Plate)	K051-H1	1x96 well plate
Tranylcypromine	X042-1EA	1EA
Triiodothyronine (T3) ELISA Kit (5 Plate)	K056-H5	5x96 well plate
Glutathione Colorimetric Detection Kit (4 Plate)	K006-H1	4x96 well plate
Cyclic GMP Direct ELISA Kit (1 Plate)	K020-H1	1x96 well plate
Progesterone ELISA Kit (5 Plate)	K025-H5	5x96 well plate
Cyclic AMP Direct ELISA Kit (5 Plate)	K019-H5	5x96 well plate
Estradiol ELISA Kit (1 Plate)	K030-H1	1x96 well plate
Oxytocin ELISA Kit (1 Plate)	K048-H1	1x96 well plate
Estrone-3-Glucuronide (E1G) ELISA Kit (5 Plate)	K036-H5	5x96 well plate
Pregnanediol-3-Glucuronide (PDG) ELISA Kit (5 Plate)	K037-H5	5x96 well plate
Hemoglobin Standard, 300UL	C037-300UL	300UL
DetectX® Estrone-3-Sulfate (E1S) Antibody, 3ML	C133-3ML	3ML
Creatinine Urinary Detection Kit (2 Plate)	K002-H1	2x96 well plate
Butyrylcholinesterase Fluorescent Activity Kit (2 Plate)	K016-F1	2x96 well plate
Corticosterone ELISA Kit, (5 Plate)	K014-H5	5x96 well plate
Cyclic GMP Direct Chemiluminescent ELISA Kit (5 Plate)	K020-C5	5x96 well plate
Superoxide Dismutase (SOD) Colorimetric Activity Kit (2 Plate)	K028-H1	2x96 well plate
Catalase Colorimetric Activity Kit (2 Plate)	K033-H1	2x96 well plate
Glutathione Fluorescent Detection Kit (1 Plate)	K006-F1	1x96 well plate
Pregnanediol-3-Glucuronide (PDG) Standard, 625UL	C128-625UL	625UL
Thyroxine (T4) ELISA Kit (5 Plate)	K050-H5	5x96 well plate
Acetylcholinesterase Fluorescent Activity Kit (2 Plate)	K015-F1	2x96 well plate
Creatinine Serum Kit (2 Plate)	KB02-H1	2x96 well plate
Hydrogen Peroxide Fluorescent Detection Kit (2 Plate)	K034-F1	2x96 well plate
Creatinine Serum Low Sample Volume Kit (384-well Plate)	KB02-H1D	1x384 well plate
Recombinant Obelin, 100UG	L001-100UG	100UG
Dry DMSO, 20ML	X022-20ML	20ML
Dry DMSO, 2ML	X022-2ML	2ML
Urea Nitrogen Standard, 1ML	C089-1ML	1ML
NADPH lyophilized, 1EA	X045-1EA	1EA
DetectX® PGE2 Conjugate, 13ML	C179-13ML	13ML
17-Hydroxyprogesterone ELISA Kits	K053-H	1 Kit
Arg8-Vasopressin (AVP) ELISA Kits	K049-H	1 Kit
Arg8-Vasopressin (AVP) Chemiluminescent ELISA Kits	K049-C	1 Kit
Androstenedione ELISA Kits	K070-H	1 Kit
Aldosterone Chemiluminescent ELISA Kits	K052-C	1 Kit
Allopregnanolone ELISA Kits	K061-H	1 Kit
Adrenocorticotropic Hormone (ACTH) ELISA Kits	K072-H	1 Kit
Alkaline Phosphatase (ALP) Colorimetric Activity Kit	K082-H1	1 Kit
3′,3′-Cyclic GAMP ELISA Kits	K073-H	1 Kit
20-Hydroxyecdysone ELISA Kit	K066-H	1 Kit
2′,3′-Cyclic GAMP STING-Based FRET Detection Kits	K081-F	1 Kit
2′,3′-Cyclic GAMP ELISA Kits	K067-H	1 Kit
Cyclic GMP Direct ELISA Kits – Improved Sensitivity	K065-H	1 Kit
Endothelin-1 (ET-1) ELISA Kit	K045-H1	1 Kit
Estrone-3-Sulfate (E1S) ELISA Kits	K038-H	1 Kit
Assay Buffer, 5X	X065-110ml	110ml
Blocking Buffer, 10X	X109-25ml	25ml
Goat anti-Rabbit IgG Coated Plate	X016-1EA
HRP Conjugate Diluent	X076-300ml	300ml
TMB Substrate	X019-55ml	55ml
Mesotocin Solution (500ng/mL)	X127-625UL
5-Azacytidine	P012-50MG	50mg

ARBOR ASSAYS 免疫測(cè)試產(chǎn)品 試劑盒-相關(guān)技術(shù)文章

ARBOR ASSAYS 免疫測(cè)試產(chǎn)品試劑盒-相關(guān)技術(shù)文章